Note: Various crosses using mutant and
recombinant strains were carried out to obtain the linkage information given above. A
complete list of strains together with the University of Nottingham culture collection
numbers is given in Table 5.1. The mutant strains were derived from
M-8650, which in turn was derived from the Brotzu isolate (Fig. 5.1).
Mutant strains were selected following treatment with far ultra-violet radiation except
for azu-1, ben-1 and pyt-2 which were recovered as spontaneous mutations.
TABLE 5.1 List of mutant and recombinant strains
of C. acremonium
| Strain genotype* |
Code** |
Comments |
| M-8650 |
20-1 |
|
| ane-5,ino-1 |
20-8 |
|
| ane-5,leu-2 |
20-9 |
|
| azu-1,arg-1,mor-1,leu-1,red-1 |
20-2 |
Obtained from Glaxochem |
| azu-1,arg-1,mor-1,leu-1,red-1,pyt-1 |
20-3 |
|
| azu-1,arg-1,mor-1,leu-1,red-1,pyt-1,ben-1 |
20-4 |
|
| azu-1,arg-2,met-1 |
20-5 |
Obtained from Glaxochem |
| lys-1,arg-6 |
20-11 |
|
| lys-1,arg-6,pyt-2,leu-3 |
20-12 |
|
| lys-1,his-1 |
20-14 |
|
| lys-1,phe-1 |
20-13 |
|
| red-1,arg-2,pyt-1 |
20-16 |
Recombinant from cross G5 |
| red-1,ino-1,azu-1,ben-1 |
20-19 |
Recombinant from cross G6 |
| red-1,lys-1,pyt-1 |
20-15 |
Recombinant from cross G1 |
| arg-1,his-1,azu-1 |
20-17 |
Recombinant from cross G1 |
| arg-1,ino-1,azu-1,ben-1,pyt-1,mor-1 |
20-18 |
Recombinant from cross G3 |
| ino-1,leu-1,ben-1,mor-1 |
20-20 |
Recombinant from cross G3 |
*Allele symbols are defined in Table 2.2
**University of Nottingham, Botany Department culture collection number
FIGURE 5.1 Genealogy of strains derived from the
Brotzu isolate
Cephalosporium acremonium (Brotzu isolate)
(IMI 49,137 = ATCC 11,550)
|
M-2072*
|
M-5016*
|
M-8650*
*Carried out at the Medical Research Council, Clevedon, England for improved production
of cephalosporin C
TABLE 2.2 Allele symbols used in this thesis
| ane |
requirement for aneurin |
| arg |
requirement for arginine |
| azu |
resistance to 6-azauracil (500 µg ml-1) |
| ben |
resistance to benlate (2.5 µg ml-1) |
| his |
requirement for histidine |
| ile |
requirement for isoleucine |
| ino |
requirement for inositol |
| leu |
requirement for leucine |
| lys |
requirement for lysine |
| met |
requirement for methionine |
| mor |
abnormal colony morphology |
| phe |
requirement for phenylalanine |
| pyt |
resistance to pyrithiamine (10 µg ml-1) |
| red |
red mycelial pigment (light dependent) |
| trp |
requirement for tryptophan |
|