Figure 4.1  Regeneration and reversion of protoplasts of C. acremonium on SMS observed using phase contrast and fluorescent microscopy illustrating the typical sequence of events.

(1) and (2) After 16 h incubation; small patches of fluorescence were clearly visible on the regenerating protoplasts.

(3) and (4) After 32 h; outgrowth of short chains of brightly fluorescing "yeast-like" cells.

(5) and (6) After 40 h; climax of protoplast reversion with the development of normal hyphae.

The bar markers represent 20 µm.