This project set out to investigate the potential application of in situ DNA hybridisation for the quantitative analysis of animal fibre blends. It was found that nuclei contained within individual immature hair follicles can be directly visualised using the technique of in situ DNA hybridisation and a high-powered microscope. As the follicle matures, the nuclei become elongated but the signal does not extend into the more mature parts of the fibre where keratinisation has taken place. The research indicated that in situ DNA analysis of intact fibres is not practically possible since the DNA is encapsulated inside the waterproof environment of the keratinised cells. Attempts to get at this DNA leads to the dissolution of the fibres. However, evidence was obtained that the technique could have application in the analysis of processed meat products.

Publications

Broadbent, E.L. (1997). PhD Thesis, University of Leeds.